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Objective:To investigate the effects of comprehensive geriatric assessment(CGA)and intervention on treatment outcomes of type 2 diabetes and concurrent emotional disorders in the elderly.Methods:108 type 2 diabetes cases with emotional disorders were enrolled from March 2020 to March 2021 in the First Affiliated Hospital of Zhengzhou University and randomly divided into the conventional treatment group and the CGA group(54 cases each). The control group was given standard drug treatment and psychological counseling, whereas the CGA group was additionally given individualized treatment based on CGA guidelines.After observation for 8 weeks, clinical effects in the two groups were examined.Changes in cognitive status, nutritional status, fall risk and results on other indicators in patients were comprehensively assessed and statistically analyzed.Results:Compared with pre-treatment data, 8 weeks of treatment for both the control group and the CGA group resulted in decreased FPG levels[control group(10.16±0.90)mmol/L, (8.70±2.98)mmol/L, t=2.58, P=0.011; CGA group: (9.94±0.82)mmol/L, (7.12±2.25)mmol/L, t=8.65, P=0.000], 2hPG levels[control group: (11.83±3.92)mmol/L, (10.53±2.70)mmol/L, t=2.01, P=0.047; CGA group: (10.64±2.99)mmol/L, (9.26±1.89)mmol/L, t=2.87, P=0.005], HbA 1C(%)[control group: (9.76±1.09)%, (8.66±2.64)%, t=2.83, P=0.006; CGA group: (9.38±2.92)%, (7.81±1.78)%, t=3.37, P=0.001], HAMD-24 scores[control group(25.69±4.70), (24.20±2.48), t=2.06, P=0.042; CGA group(24.03±4.00), (22.11±1.38), t=3.33, P=0.001], and HAMD-14 scores[control group(19.66±2.84), (18.41±2.34), t=2.50, P=0.014; CGA group(18.77±2.48), (17.39±2.36), t=2.96, P=0.004], respectively, with more marked changes in all measures in the CGA group than in the control group(all P<0.05). Conclusions:Early CGA can effectively improve blood glucose levels and relieve mood disorders in elderly type 2 diabetes with emotional disorders.
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Objective:To explore the pathway of psychological capital and burnout on turnover intention of nurses in hematopoietic stem cell transplantation ward.Methods:The cross-sectional research design involved 180 registered nurses in hematopoietic stem cell transplantation wards from 16 tertiary grade A general hospitals with hematopoietic stem cell transplantation qualifications in Shandong province in October 2020. SPSS 22.0 was used to analyze the data, and Bootstrap method was used to test the mediating effect of psychological capital between burnout and turnover intention.Results:The average scores of psychological capital, burnout and turnover intention of nurses in hematopoietic stem cell transplantation ward were (4.70 ± 0.73), (2.44 ± 0.88) and (2.32 ± 0.68), respectively. There were significant differences in turnover intention among nurses in hematopoietic stem cell transplantation ward at different ages, transplant nursing age, marital status and night shift ( F=3.04, 3.34, t=2.43, -3.12, P<0.05); psychological capital was negatively correlated with burnout and turnover intention ( r=-0.698, -0.450, P<0.01), while burnout was positively correlated with turnover intention ( r=0.512, P<0.01) ; optimism in psychological capital partially mediated the relationship between burnout and turnover intention, and the mediating effect accounted for 52.7% of the total effect. Conclusions:The turnover intention of nurses in hematopoietic stem cell transplantation ward was at a high level, and psychological capital had a certain impact on turnover intention. Administrators should formulate targeted strategies to improve psychological capital, reduce the burnout and turnover intention of nurses in hematopoietic stem cell transplantation ward, in order to form a stable and high-quality team of nursing personnel for hematopoietic stem cell transplantation, and promote the development of transplantation nursing specialty in China.
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Objective To investigate the prevalence and features of thyroid nodules in middle and aged patients with type 2 diabetes mellitus(T2DM). Methods High-resolution ultrasonography was used to detect thyroid nodules in 132 cases middle and aged patients with type 2 diabetes and 89 patients without diabetes.The nodule features and its relationships with related indicators in diabetic patients were analyzed. Results The prevalence of thyroid nodules in middle and aged patients with type 2 diabetes was higher than that without diabetes (67.4％ vs. 53.9％,P＜0.05),and most occurred in 50 to 59 age group (66.7％ vs. 42.9％) without dependence on changes in thyroid functions and volumes.In diabetes group,the prevalence of thyroid nodules were 59.5％ in male and 81.3％ in female (P＜0.05),no obvious difference was observed in the size and number of thyroid nodules between male and female,multiple nodules and micronodule (＜ 1.0 cm) had the higher incidences in both sexes.The prevalence of thyroid nodules was increased with aging,but not with diabetes duration and glycosylated hemoglobin (HbAlc) level (x2 =0.797,P=0.372; x2 =1.078,P =0.229). Conclusions It is common that thyroid nodules combined with diabetes in middle and aged patients,thyroid ultrasound screening and regular following-up of patients aged ≥50 years have important clinical significance.
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Objective To investigate the characteristics of the blood glucose fluctuation in elderly patients with type 2 diabetes mellitus (T2DM). Methods The 92 elderly patients with T2DM (the elderly group) and 58 young and middle-aged patients with T2DM (the non-elderly group) were monitored using the continuous glucose monitoring system(CGMS). The characteristics of glucose profiles of the two different age groups, and of the different glycosylated hemoglobin (HbA1c) level groups in the elderly were comparatively analyzed. Results (1)There was no significant difference in HbA1c level between the elderly group and the non-elderly group. Compared with the non-elderly group, the elderly group showed the increases in blood glucose fluctuant coefficient [BGFC, (2.68±1.00) mmol/L vs. (2.12±0.74) mmol/L, t=-3.691, P＜0.001], in postprandial glucose excursion (PPGE) of breakfast and supper [(5.96±2.47) mmol/L vs. (5.11±2.44) mmol/L, t=-2.058, P＜0.05; (5.17±2.15) mmol/L vs. (4.16±2.28) mmol/L, t=-2.730, P＜0.01], in the time to postprandial glucose peak of breakfast and lunch [(112.5±29.7) min vs. (97.0±27.2) min, t=-3.225, P＜0.01; (140.0±39.7) min vs. (118.1±42.6) min, t=-3.195, P＜0.01], in the frequency of hypoglycemia (26.3% vs. 5.5%, P＜0.05), and showed the largest amplitude of glycemic excursions [LAGE, (9.66±2.48) mmol/L vs.(8.40±3.13) mmol/L, t=-2.720, P＜0.01]. (2)In the elderly, along with decreased HbA1c, the incidence of hypoglycaemia increased (P＜0.05); And along with increased HbA1c, the amplitude of blood glucose fluctuation increased. There were significant differences in BGFC, PPGE of breakfast and lunch, and LAGE among different HbA1c level groups (P＜0.01, P＜0.05, P＜0.05, P＜0.001). (3)HbA1c was positively correlated with FBG, mean blood glucose (MBG), percentage of time at glycemia (PT7.8, PT11.1), the lowest blood glucose (LBG), the highest blood glucose (HBG), BGFC, PPGE and LAGE (r=0.899-0.289, all P＜0.001). Multiple stepwise regression analysis indicated that MBG, FBG and PT7.8 was the independent influential factor of HbA1c (adjusted R2=0.807, P＜0.05). Conclusions The elderly patients with T2DM are at a particularly high risk for postprandial hyperglycemia and nocturnal hypoglycemic episodes, CGMS could show glucose fluctuation characters of T2DM patients diurnally, and provide a clinical basis for reasonable therapy.
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This study was aimed to examine the expression of apoptosis-associated gene Fas in HeLa cell, explore the effects of the co-immobilized cytokines (tumor necrosis factor-alpha and interferon-gamma), and probe the potential mechanism of action. The preparation and application of the research couple IFN-gamma and TNF-alpha to the polystyrene cell culture plate were performed using the Photo-immobilization method, with different doses (20 ng/well and 200 ng/well) and synthesized optical active material. HeLa cells were treated with cytokines for two dose and 1, 3, 6 days. The result showed that the free cytokines induced HeLa apoptosis quickly, yet the HeLa apoptosis induced by co-immobilized cytokines had longer effect.
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Humans , Apoptosis , Genetics , Drug Synergism , HeLa Cells , Immobilized Proteins , Chemistry , Pharmacology , Interferon-gamma , Chemistry , Pharmacology , Tumor Necrosis Factor-alpha , Chemistry , Pharmacology , Up-Regulation , fas Receptor , MetabolismABSTRACT
Objective:To observe the effect of triplex forming oligonucleotide(TFO) of PDGF-B chain on cell proliferation and cell cycle of rat glioma.Methods:1?10 6 C 6 glioma cells with high-flow microinfusion were seeded into right caudate putamen of rats.TFO was used in situ a week after glioma cell inoculation.The treatment groupⅠandⅡwere treated with 1.5 mg/20 ?l and 3.0 mg/20 ?l TFO respectively on 8,11 and 14 d after cell inoculation.The control group was treated with 20 ?l normal saline with the same procedure.Three weeks after cell inoculation all rats were killed and samples were detected with macroscopic,microscopic histology and immunofluorescence flow cytometric analysis.Results:The inhibition rates of tumor growth were 66.1% in the treatment groupⅠand 91.8% in the treatment group Ⅱ.TFO specifically blocked expressions of PDGF-B and PCNA of glioma cells in a concentration-dependent fashion. TFO obviously inhibited the transit of cells from the G 0-G 1 to S phase in a concentration-dependent fashion.Conclusion:TFO can commendably block PDGF-B expression,inhibit cell proliferation and induce C 6 glioma cells arrest in the G 1 phase,and thus inhibit tumor growth of glioma.
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Objective To observe the inhibit effect of triplex forming oligonucleotide (TFO)(platelet-derived growth factor-B chain, PDGF) on tumor growth and angiogenesis in rats with glioma.Methods 1?10~6 C_6 glioma cells with high-flow microinfusion were seed into right caudate putamens of 18 rats by stereotaxic technique. TFO was injected in situ 1 week after glioma cells inoculation. Treat group Ⅰ and treat group Ⅱ received TFO at dose of 1.5 mg/20 ?l and 3.0 mg/20 ?l, respectively. The same doses were given again at 8, 11 and 14th day after glioma cells inoculation. The control group was treated with 20 ?l normal saline at same time like treat groups. Three weeks after glioma cells inoculation, all the rats were killed. The expressions of, PDGF-B, vascular endothelial growth factor (VEGF) and proliferating cell nuclear antigen (PCNA) were detected with microscopic histology.Results The inhibition rate of tumor growth was 66.0% in treat groupⅠand 92.2% in treat group Ⅱ. There was significant difference between the two groups ((P
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To observe the inhibitory effects of VEGF antisense oligonuoleotide on angiogenesis and glioma growth of tumor bearing rats. 1?10 6 C 6 glioma cells(20?l) were seeded with high flow microinfusion into right caudate nucleus of all rats with sterotactic technique. In the treatment groups Ⅰ and Ⅱ 1 000 ?mol/L, 2 000 ?mol/L VEGF antisense oligonucleotide was respectively given at the site of seeding twice 1 and 2 weeks after cell inoculation. The control group received only 20?l Hank′s solution twicely. Three weeks after cell inoculation, the general condition of the treated rats were better, while rats in control group were in precarious conditions. The inhibition rate of tumor growth was 91 5% in the treated group Ⅰ, 100% in the treated group Ⅱ. The expressions of VEGF mRNA, VEGF, and MVD were significantly different between treatment and control groups. The results indicated that VEGF was required for the maintenance of angiogenesis in the tumor, and inhibition of VEGF might be a therepeutic strategy in the treatment of glioma.
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Objective:To observe the inhibitory effect of triplex forming oligonucleotede(TFO) of PDGF-B chain combined with antisense oligonucleotide(AON) of VEGF on glioma growth in rats.Methods: Thirty-six male SD rats received 1?10~(6) C_(6) glioma cells/20 ?l normal saline into the right caudate putamen by stereotactic technique.GroupⅠ(n=6) was treated in situ with 1.5 mg/20 ?l TFO for 3 times on 8,11 and 14 d after glioma cell inoculation.Group Ⅱ(n=12) and Ⅲ(n=12) were respectively treated with TFO 1.5 mg+AON 0.125 mg/20 ?l and TFO 1.5 mg+AON 0.250 mg/20 ?l for 3 times on 8,11 and 14 d after glioma cell inoculation.Control group(n=6) was treated with 20 ?l saline for 3 times at the same time pionts.Three weeks after cell inoculation,all rats were sacrificed to observe tumor growth and to determine the expression of PDGF-B,VEGF and PCNA.Results: The inhibition rate of tumor growth was 53.1% in groupⅠ,81.4% in group Ⅱ and 93.1% in group Ⅲ,with significant difference found between the 3 groups(P
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To observe the inhibition of vascular endothelial growth factor(VEGF) expression in C 6 glioma cells with VEGF antisense oligonucleotides.VEGF expression in C 6 glioma cells was examined quantitatively and qualitatively with immunofluorescence flow cytometric analysis and immunohistochemistry.Results showed that VEGF antisense ODNs inhibited the VEGF expression in C 6 glioma cells,and the inhibition was concentration dependent.This indicates that VEGF antisense ODNs can effectively suppress the VEGF expression in C 6 glioma cells.